The brown citrus aphid, Toxoptera citricida (Kirkaldy), is one of the most devastating pests of citrus, causing extensive crop losses worldwide. These data will aid research efforts in the identification of important genes within insects, specifically aphids and other sap feeding insects within the Order Hemiptera. Sequences returning matches with an E-value of ≤ −10 using BLASTX, BLASTN, or TBLASTX were annotated based on their putative molecular function and biological process using the Gene Ontology classification system.
Approximately 33% of the ESTs currently have no significant match in either the non-redundant protein or nucleic acid databases.
Contig alignment of these ESTs resulted in 2124 total assembled sequences, including both contiguous sequences and singlets. Over 5180 cDNA clones were sequenced, resulting in 4263 high-quality ESTs. The cDNA library was derived from alate adults due to their significance in spreading viruses (e.g., citrus tristeza virus). In this paper, we describe the first public data set of ESTs from the brown citrus aphid, T. However, important morphogenetic processes are exclusively associated with piercing-sucking insect development and sap feeding insect metabolism. Although there is a growing database in GenBank of ESTs from insects, most are from Drosophila melanogaster and Anopheles gambiae, with relatively few specifically derived from aphids. Similar large-scale expressed sequence tag (EST) sequencing projects from other insects have provided a vehicle for answering biological questions relating to development and physiology. To better understand the biology of the brown citrus aphid and the emergence of genes expressed during wing development, we undertook a large-scale 5′ end sequencing project of cDNA clones from alate aphids. The alate (winged) form of this aphid can readily fly long distances with the wind, thus spreading citrus tristeza virus in citrus growing regions. The brown citrus aphid, Toxoptera citricida (Kirkaldy), is considered the primary vector of citrus tristeza virus, a severe pathogen which causes losses to citrus industries worldwide.
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